Schiff base
R
1
N=CH-CH=CH-NHR
2
free radicals
lipid (PUFA)
protein
nucleic acid
+
Lipofuscin
O
2
OH
Biochemical method
Excite
Emit
Age Pigments (Lipofuscin)
Em wavelength
(nm)
Fluorocence intensity
Blue crab
krill
Age Pigments (Lipofuscin) formation